Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces

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dc.contributor.orgDepartment of Biosciences
dc.creatorAmeruoso, Andrea
dc.date.accessioned2021-10-27T14:01:41Z
dc.date.available2021-10-27T14:01:41Z
dc.date.issued2021-10-27
dc.description.abstractThe rise of antibiotic-resistant bacteria represents a major threat to global health, creating an urgent need to discover new antibiotics. Natural products derived from the genus Streptomyces represent a rich and diverse repertoire of chemical molecules from which new antibiotics are likely to be found. However, a major challenge is that the biosynthetic gene clusters (BGCs) responsible for natural product synthesis are often poorly expressed under laboratory culturing conditions, thus preventing isolation and screening of novel chemicals. To address this, we describe a novel approach to activate silent BGCs through rewiring endogenous regulation using synthetic gene regulators based upon CRISPR-Cas. First, we create CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) systems that allow for highly programmable and effective gene repression and activation in Streptomyces. We then harness these tools to activate a silent BGC through perturbing its endogenous regulatory network. Together, this work advances the synthetic regulatory toolbox for Streptomyces and facilitates the programmable activation of silent BGCs for novel chemical discovery.
dc.format.mimetypeapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheet
dc.identifier.citationAmeruoso, Andrea (2021): Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces. [Dataset]. Rice University. https://doi.org/10.25611/5SAC-CW45.
dc.identifier.doihttps://doi.org/10.25611/5SAC-CW45
dc.identifier.urihttps://hdl.handle.net/1911/111612
dc.language.isoeng
dc.publisherRice University
dc.rightsCC0 1.0 Universal
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/
dc.subject.keywordStreptomyces
dc.subject.keywordbiosynthetic gene clusters
dc.subject.keywordCRISPRi
dc.subject.keywordCRISPRa
dc.subject.keywordnatural products
dc.titleActivating natural product synthesis using CRISPR interference and activation systems in Streptomyces
dc.title.subtitleDataset
dc.type.dcmiDataset
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