Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375

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dc.contributor.advisorWilson, Lon J.en_US
dc.creatorBerger, Christopher Scotten_US
dc.date.accessioned2011-07-25T02:07:09Zen_US
dc.date.available2011-07-25T02:07:09Zen_US
dc.date.issued2010en_US
dc.description.abstractFor the first time, C60-monoclonal antibody (mAb) immunoconjugates have been determined to internalize into target cells using water-soluble Gd3+-ion-filled fullerenes (Gd C60(OH) x). Separate conjugations of Gd C60(OH)x with the antibody ZME-018 and a murine antibody mixture (MuIgG) took place in a 1:5 mAb:Gd 60 ratio. Quanitative analysis of the immunoconjugates was established using inductively-coupled plasma mass spectrometry (ICP-MS) and UV-Vis spectrometry (Gd C60+C60). Enzyme-linked immunosorbent assays (ELISA) show little change in the specific binding of the ZME-018 once conjugated. Each immunoconjugate was exposed to two cancer cell lines, A375m (a ZME-018-specific line), and T24, a bladder carcinoma line. Internalization of the immunoconjugate was measured at various timepoints, after which the cells were harvested and digested with 25% HClO3 for Gd3+ analysis by ICP-MS. ICP-MS results show immunoconjugate internalization peaked in the first hour of exposure of the cells with a large dropoff occuring in the subsequent hour. These are the first results demonstrating the practicality of a cancer therapy based on Fullerene Immunotherapy (FIT).en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS CHEM. 2010 BERGERen_US
dc.identifier.citationBerger, Christopher Scott. "Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375." (2010) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/62174">https://hdl.handle.net/1911/62174</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/62174en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectChemistryen_US
dc.subjectInorganic chemistryen_US
dc.titleGadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375en_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemistryen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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