Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin

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dc.citation.firstpage137
dc.citation.journalTitleJournal of Biological Inorganic Chemistry
dc.citation.lastpage144
dc.citation.volumeNumber18
dc.contributor.authorMusatov, Andrej
dc.contributor.authorFabian, Marian
dc.contributor.funderNational Institutes of Health
dc.date.accessioned2013-03-29T20:06:37Z
dc.date.available2014-03-30T05:10:04Z
dc.date.issued2013
dc.description.abstractThe interaction of peroxidized cardiolipin with ferrocytochrome c induces two kinetically and chemically distinct processes. The first is a rapid oxidation of ferrocytochrome c, followed by a slower, irreversible disruption of heme c. The oxidation of ferrocytochrome c by peroxidized cardiolipin is explained by a Fenton-type reaction. Heme scission is a consequence of the radical-mediated reactions initiated by the interaction of ferric heme iron with peroxidized cardiolipin. Simultaneously with the heme c disruption, generation of hydroxyl radical is detected by EPR spectroscopy using the spin trapping technique. The resulting apocytochrome c sediments as a heterogeneous mixture of high aggregates, as judged by sedimentation analysis. Both the oxidative process and the destructive process were suppressed by nonionic detergents and/or high ionic strength. The mechanism for generating radicals and heme rupture is presented.
dc.embargo.terms1 yearen_US
dc.identifier.citationMusatov, Andrej and Fabian, Marian. "Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin." <i>Journal of Biological Inorganic Chemistry,</i> 18, (2013) 137-144. http://dx.doi.org/10.1007/s00775-012-0958-0.*
dc.identifier.doihttp://dx.doi.org/10.1007/s00775-012-0958-0
dc.identifier.grantIDGM0843348 (National Institutes of Health)
dc.identifier.urihttps://hdl.handle.net/1911/70863
dc.language.isoeng
dc.subject.keywordferrocytochrome c
dc.subject.keywordperoxidized cardiolipin
dc.subject.keywordheme destruction
dc.subject.keywordhydroxyl radical
dc.subject.keywordaggregation
dc.titleElucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin
dc.typeJournal article
dc.type.dcmiText
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