Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin

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dc.citation.firstpage137en_US
dc.citation.journalTitleJournal of Biological Inorganic Chemistryen_US
dc.citation.lastpage144en_US
dc.citation.volumeNumber18en_US
dc.contributor.authorMusatov, Andrejen_US
dc.contributor.authorFabian, Marianen_US
dc.contributor.funderNational Institutes of Healthen_US
dc.date.accessioned2013-03-29T20:06:37Zen_US
dc.date.available2014-03-30T05:10:04Zen_US
dc.date.issued2013en_US
dc.description.abstractThe interaction of peroxidized cardiolipin with ferrocytochrome c induces two kinetically and chemically distinct processes. The first is a rapid oxidation of ferrocytochrome c, followed by a slower, irreversible disruption of heme c. The oxidation of ferrocytochrome c by peroxidized cardiolipin is explained by a Fenton-type reaction. Heme scission is a consequence of the radical-mediated reactions initiated by the interaction of ferric heme iron with peroxidized cardiolipin. Simultaneously with the heme c disruption, generation of hydroxyl radical is detected by EPR spectroscopy using the spin trapping technique. The resulting apocytochrome c sediments as a heterogeneous mixture of high aggregates, as judged by sedimentation analysis. Both the oxidative process and the destructive process were suppressed by nonionic detergents and/or high ionic strength. The mechanism for generating radicals and heme rupture is presented.en_US
dc.embargo.terms1 yearen_US
dc.identifier.citationMusatov, Andrej and Fabian, Marian. "Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin." <i>Journal of Biological Inorganic Chemistry,</i> 18, (2013) 137-144. http://dx.doi.org/10.1007/s00775-012-0958-0.en_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00775-012-0958-0en_US
dc.identifier.grantIDGM0843348 (National Institutes of Health)en_US
dc.identifier.urihttps://hdl.handle.net/1911/70863en_US
dc.language.isoengen_US
dc.subject.keywordferrocytochrome cen_US
dc.subject.keywordperoxidized cardiolipinen_US
dc.subject.keywordheme destructionen_US
dc.subject.keywordhydroxyl radicalen_US
dc.subject.keywordaggregationen_US
dc.titleElucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipinen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
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