A maverick in the pectin methylesterase family: PME31 acts in lipid droplet utilization

Simple item page
dc.contributor.advisorBartel, Bonnieen_US
dc.contributor.advisorBraam, Janeten_US
dc.creatorHamade, Sarahen_US
dc.date.accessioned2024-08-30T18:30:53Zen_US
dc.date.created2024-08en_US
dc.date.issued2024-08-06en_US
dc.date.submittedAugust 2024en_US
dc.date.updated2024-08-30T18:30:53Zen_US
dc.descriptionEMBARGO NOTE: This item is embargoed until 2030-08-01en_US
dc.description.abstractIn plants, the primary form of energy stored form in seed lipid droplets, triacylglycerol (TAG), is catabolized during germination to support pre-photosynthetic growth. While this process is essential for seedling development, it is incompletely understood. In a screen for Arabidopsis thaliana mutants with delayed lipid droplet coat protein degradation, five independent mutations in PECTIN METHYLESTERASE31 (PME31) were recovered. In addition to delayed coat protein degradation, pme31 mutant seedlings exhibited sustained lipid droplets and elevated levels of several TAG and diacylglycerol species. Although structural prediction classified PME31 as a pectinesterase, it also resembled the putative E.coli lipase, YbhC. A fluorescent PME31 reporter was cytosolic and associated with peroxisomes, the site of fatty acid catabolism, during lipid mobilization. These findings suggest that, in contrast to most PMEs, which modify cell wall pectin, PME31 functions in lipid mobilization at the peroxisome.en_US
dc.embargo.lift2030-08-01en_US
dc.embargo.terms2030-08-01en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationHamade, Sarah. A maverick in the pectin methylesterase family: PME31 acts in lipid droplet utilization. (2024). Masters thesis, Rice University. https://hdl.handle.net/1911/117828en_US
dc.identifier.urihttps://hdl.handle.net/1911/117828en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectArabidopsis germinationen_US
dc.subjectlipid dropleten_US
dc.subjectlipid mobilizationen_US
dc.subjectpectin methylesteraseen_US
dc.subjectoleosinen_US
dc.subjectperoxisomeen_US
dc.titleA maverick in the pectin methylesterase family: PME31 acts in lipid droplet utilizationen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
Files
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
PROQUEST_LICENSE.txt
Size:
5.84 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
LICENSE.txt
Size:
2.98 KB
Format:
Plain Text
Description:
Download
Collections
Rice University Theses and Dissertations