Initiation of RNA polymerization and polymerase encapsidation by a Picobirnavirus

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dc.contributor.advisorTao, Yizhi Janeen_US
dc.contributor.committeeMemberStewart, Charles Ren_US
dc.contributor.committeeMemberOlson, John Sen_US
dc.contributor.committeeMemberPrasad, B.V. Ven_US
dc.creatorCollier, Aaron Michaelen_US
dc.date.accessioned2016-01-07T17:23:01Zen_US
dc.date.available2016-01-07T17:23:01Zen_US
dc.date.created2014-12en_US
dc.date.issued2015-01-15en_US
dc.date.submittedDecember 2014en_US
dc.date.updated2016-01-07T17:23:01Zen_US
dc.description.abstractDuring the replication cycle of double-stranded (ds) RNA viruses, the viral RNA-dependent RNA polymerase (RdRP) replicates and transcribes the viral genome from within the viral capsid. How these RdRPs molecules are packaged within the virion and how they function within the confines of an intact capsid are intriguing questions that have highly variable answers depending on the virus family being examined. In this study, we have determined a 2.4 Å resolution structure of an RdRP from a human infecting strain of picobirnavirus (PBV). In addition to a conserved polymerase fold, the PBV RdRP possesses a unique, highly flexible 24-aa loop structure (aa495-518) located near the C-terminus of the protein that is inserted into its active site. In vitro RNA polymerization assays have shown that the wild-type RdRP is capable of initiating RNA synthesis using a de novo mechanism, while a mutant RdRP lacking the loop structure could only synthesize RNA through back-priming, suggesting that the loop likely functions as a platform for the priming nucleotide to bind. Unexpectedly, co-expression of the PBV RdRP with its respective capsid protein (CP) indicated that the PBV RdRP could not be incorporated into recombinant capsids in the absence of the viral genome. Additionally, the PBV RdRP exhibited a high affinity towards the conserved 5’-terminal sequence of the viral genome, suggesting that PBV RdRP molecules are likely packaged through their specific binding to viral RNA during assembly.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationCollier, Aaron Michael. "Initiation of RNA polymerization and polymerase encapsidation by a Picobirnavirus." (2015) Diss., Rice University. <a href="https://hdl.handle.net/1911/87757">https://hdl.handle.net/1911/87757</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/87757en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectdouble-stranded RNA virusen_US
dc.subjectRNA-dependent RNA polymeraseen_US
dc.subjectvirus assemblyen_US
dc.subjectpicobirnavirusen_US
dc.subjectgastroenteritisen_US
dc.subjectRNA replicationen_US
dc.subjectRNA transcriptionen_US
dc.subjectinitiation of RNA polymerizationen_US
dc.subjectback-primingen_US
dc.subjectterminal nucleotidyl transferaseen_US
dc.titleInitiation of RNA polymerization and polymerase encapsidation by a Picobirnavirusen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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