The Folding and Binding Partners of the Perlecan SEA Module

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dc.contributor.advisorMcNew, James A.en_US
dc.contributor.committeeMemberFarach-Carson, Cindyen_US
dc.contributor.committeeMemberCarson, Daniel D.en_US
dc.contributor.committeeMemberSegatori, Lauraen_US
dc.creatorDiaz, Arielen_US
dc.date.accessioned2012-09-06T04:30:01Zen_US
dc.date.accessioned2012-09-06T04:30:05Zen_US
dc.date.available2012-09-06T04:30:01Zen_US
dc.date.available2012-09-06T04:30:05Zen_US
dc.date.created2012-05en_US
dc.date.issued2012-09-05en_US
dc.date.submittedMay 2012en_US
dc.date.updated2012-09-06T04:30:05Zen_US
dc.description.abstractSperm protein, enterokinase and agrin (SEA) modules are small folds within large heavily glycosylated modular proteins. Because decreased expression of SEA-containing proteins such as perlecan (PLN) can lead to diseases such as Schwartz-Jampel syndrome (SJS), characteristics of the PLN SEA module including folding, potential for autocleavage, and protein binding were studied. Sequence analyses, recombinant protein evaluation, and a yeast two-hybrid screen were used to study the PLN SEA module and compare it to the mucin (MUC) 1 SEA module. In silico modeling of the PLN SEA module demonstrated a well conserved α/β sandwich fold. Experiments with expressed proteins showed that unlike MUC1, the PLN SEA module does not autocleave. Two-hybrid screening identified four “high confidence” proteins as potential binding partners which were explored in preliminary experiments. Together, these results demonstrate that PLN SEA module is unique and its properties cannot be generalized with other SEA module proteins such as MUC1.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationDiaz, Ariel. "The Folding and Binding Partners of the Perlecan SEA Module." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/64678">https://hdl.handle.net/1911/64678</a>.en_US
dc.identifier.slug123456789/ETD-2012-05-144en_US
dc.identifier.urihttps://hdl.handle.net/1911/64678en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectSEA moduleen_US
dc.subjectSEA domainen_US
dc.subjectPerlecanen_US
dc.subjectHSPG2en_US
dc.subjectAutocleavageen_US
dc.subjectAutoproteolysisen_US
dc.subjectMUC1en_US
dc.subjectSchwartz-Jampel syndromeen_US
dc.titleThe Folding and Binding Partners of the Perlecan SEA Moduleen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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