Uncovering the signal transduction pathway that regulates the proliferation of Dictyostelium cells

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dc.contributor.advisorGomer, Richard H.en_US
dc.creatorHanson, Nanaen_US
dc.date.accessioned2011-07-25T02:05:26Zen_US
dc.date.available2011-07-25T02:05:26Zen_US
dc.date.issued2010en_US
dc.description.abstractAprA (Autocrine Proliferation Repressor) is a secreted factor that represses proliferation in the slime mold Diclyostelium discoideum . Although the role AprA plays in the regulation of proliferation is known, the signaling pathway by which it occurs is yet to be determined. To discover the different components of the AprA signaling pathway, I examined 12 REMI mutants which proliferated in a similar manner to aprA - cells. Three of 12 mutants were insensitive to AprA. MetPOE was one of the mutants that were insensitive to AprA, and I attempted to construct a complete knockout of the disrupted gene. From an additional REMI screen that I conducted, I found that disruption of dokA led to a mutant with a phenotype similar to aprA-, suggesting this protein may also have a role in the AprA signal transduction pathway. Preliminary data suggested that G proteins may also play a role in the AprA signal transduction pathway. I further characterized six G protein null mutants. I determined that both galpha8- and gbeta- cells were multinucleate. Also there was an increase in the percentage of cells in S phase in gbeta - cells. The similarities between aprA- cells and ga8- and gbeta - cells suggested that Galpha8- and Gbeta - may be part of the AprA signal transduction pathway.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS BIOCHEM. 2010 HANSONen_US
dc.identifier.citationHanson, Nana. "Uncovering the signal transduction pathway that regulates the proliferation of Dictyostelium cells." (2010) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/62027">https://hdl.handle.net/1911/62027</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/62027en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectCellular biologyen_US
dc.subjectChemistryen_US
dc.subjectBiochemistryen_US
dc.titleUncovering the signal transduction pathway that regulates the proliferation of Dictyostelium cellsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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