Analyzing Single-Molecule Manipulation Experiments

dc.contributor.authorCalderon, Christopher P.en_US
dc.contributor.authorHarris, Nolan C.en_US
dc.contributor.authorKiang, Ching-Hwaen_US
dc.contributor.authorCox, Dennis D.en_US
dc.date.accessioned2018-06-19T17:13:03Zen_US
dc.date.available2018-06-19T17:13:03Zen_US
dc.date.issued2008-10en_US
dc.date.noteOctober 2008en_US
dc.description.abstractSingle-molecule manipulation studies can provide quantitative information about the physical properties of complex biological molecules without ensemble artifacts obscuring the measurements. We demonstrate computational techniques which aim at more fully utilizing the wealth of information contained in noisy experimental time series. The "noise" comes from multiple sources, e.g. inherent thermal motion, instrument measurement error, etc. The primary focus of this article is a methodology for using time domain based methods for extracting the effective molecular friction from single-molecule pulling data. We studied molecules composed of 8 tandem repeat titin I27 domains, but the modeling approaches have applicability to other single-molecule mechanical studies. The merits and challenges associated with applying such a computational approach to existing single-molecule manipulation data are also discussed.en_US
dc.format.extent8 ppen_US
dc.identifier.citationCalderon, Christopher P., Harris, Nolan C., Kiang, Ching-Hwa, et al.. "Analyzing Single-Molecule Manipulation Experiments." (2008) <a href="https://hdl.handle.net/1911/102107">https://hdl.handle.net/1911/102107</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/102107en_US
dc.language.isoengen_US
dc.titleAnalyzing Single-Molecule Manipulation Experimentsen_US
dc.typeTechnical reporten_US
dc.type.dcmiTexten_US
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