Effect of laminar shear stress on gene regulation, protein synthesis, and protein secretion by cultured human endothelial cells
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To test the hypothesis that wall shear stress generated by blood flow may regulate endothelial cell expression of blood clot dissolving proteins or vasoactive proteins, an in vitro perfusion system was used to expose human umbilical vein endothelial cell monolayers to well defined, laminar fluid flow. Protein production studies utilized immunoassays, while semi-quantitative studies of messenger RNA levels in a small numbers of cells required a reverse transcription/polymerase chain reaction technique.
Secretion by endothelial cells of the two main regulators of the fibrinolytic (ie blood clot dissolving) system, tissue plasminogen activator (tPA) and plasminogen activator inhibitor, type 1 (PAI-1) were not affected by exposure to venous levels of shear stress (4 dynes/cm
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Diamond, Scott Lee. "Effect of laminar shear stress on gene regulation, protein synthesis, and protein secretion by cultured human endothelial cells." (1990) Diss., Rice University. https://hdl.handle.net/1911/16336.