Endothelial cell and mural progenitor cell co-culture to promote angiogenesis in tissue engineered constructs

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dc.contributor.advisorWest, Jennifer L.
dc.creatorSmith, April Ann
dc.date.accessioned2018-12-03T18:31:17Z
dc.date.available2018-12-03T18:31:17Z
dc.date.issued2008
dc.description.abstractThe need for tissue engineered organs is great since only 15% of those on the transplant waiting list received organs in 2006. The ability to vascularize a tissue engineered construct is one of the major hurdles for tissue engineering because it can take 2 weeks for an implanted construct to become vascularized. To produce prevascularized tissues, the cellular interactions that occur during vascular formation need to be examined. In this work, human umbilical cord endothelial cells (HUVECs) and mural progenitor cells (10T1/2 cells) are cultured together for 6 days. Western blotting and immunofluorescence are performed to assess SM-specific marker expression. αSM-actin and calponin expression was observed in co-cultures seeded at 300,000 cells/well while caldesmon and SMMHC expression was observed in co-cultures seeded at 60,000 cells/well. HUVECs and 10T1/2 cells, seeded at 150,000 cells/gel, encapsulated in degradable PEG hydrogels expressed high levels of αSM-actin, calponin, and caldesmon.
dc.format.extent51 pp
dc.identifier.callnoTHESIS BIOENG. 2009 SMITH
dc.identifier.citationSmith, April Ann. "Endothelial cell and mural progenitor cell co-culture to promote angiogenesis in tissue engineered constructs." (2008) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/103542">https://hdl.handle.net/1911/103542</a>.
dc.identifier.digital304508007
dc.identifier.urihttps://hdl.handle.net/1911/103542
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectBiomedical engineering
dc.subjectApplied sciences
dc.titleEndothelial cell and mural progenitor cell co-culture to promote angiogenesis in tissue engineered constructs
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBioengineering
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
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