A multi-photon microscope for three dimensional functional recording of fast neuronal activity

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dc.contributor.advisorSaggau, Peteren_US
dc.creatorReddy, Gaddum Duemanien_US
dc.date.accessioned2009-06-03T21:10:11Zen_US
dc.date.available2009-06-03T21:10:11Zen_US
dc.date.issued2007en_US
dc.description.abstractA thorough understanding of how neurons work is one of the greatest scientific goals in the field of experimental neuroscience. However, four fundamental technical limitations complicate any attempt to study neuronal function with sub-cellular resolution: First, neurons and neuronal processes are small, second, in realistic experimental situations they can be located deep within optically scattering tissue, third, the chemical and electrical signaling that characterizes neuronal behavior happens quickly, and fourth, neurons and neuronal processes have very three dimensional (3D) shapes. Here we develop a tool that overcomes all four listed limitations by combining the technique of multi-photon microscopy with a unique method for 3D laser beam steering. The result is an instrument capable of monitoring physiological signals at multiple locations in the volume of space occupied by a neuron, a task that is unachievable with any other available instrument.en_US
dc.format.extent153 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS BIOENG. 2007 REDDYen_US
dc.identifier.citationReddy, Gaddum Duemani. "A multi-photon microscope for three dimensional functional recording of fast neuronal activity." (2007) Diss., Rice University. <a href="https://hdl.handle.net/1911/20637">https://hdl.handle.net/1911/20637</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/20637en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectNeurosciencesen_US
dc.subjectBiomedical engineeringen_US
dc.titleA multi-photon microscope for three dimensional functional recording of fast neuronal activityen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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