The role of sampling location and depth in the performance of lake plant environmental DNA metabarcoding

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dc.contributor.advisorCorrea, Adrienne MSen_US
dc.contributor.advisorEgan, Scott Pen_US
dc.creatorDrummond, Jenn Aen_US
dc.date.accessioned2020-04-27T19:28:37Zen_US
dc.date.available2020-04-27T19:28:37Zen_US
dc.date.created2020-05en_US
dc.date.issued2020-04-24en_US
dc.date.submittedMay 2020en_US
dc.date.updated2020-04-27T19:28:37Zen_US
dc.description.abstractInventories of organisms are central to ecological research and to ecosystem management. Environmental DNA (eDNA) methods permit broad yet detailed biodiversity sampling to be performed with minimal field effort, but uncertainty remains regarding the spatial resolution necessary for effective sampling. We analyze eDNA samples from twelve temperate lakes via ITS1 rRDNA metabarcoding for aquatic and terrestrial plant taxonomic patterns across horizontal and vertical space. We detect the expected community variation (beta diversity) from lake to lake, but few community composition patterns related to offshore distance and sampling depth. Patterns included slightly elevated alpha diversity in near-shore samples, but no other significant effects. Read abundances were heavily weighted toward aquatic macrophytes, but we identified representatives of many other phylogenetically and environmentally varied taxa, including terrestrial species from surrounding areas. We conclude that freshwater plant eDNA surveys successfully capture differences among lake communities, allowing easily-accessible shore-based sampling to inform research and management.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationDrummond, Jenn A. "The role of sampling location and depth in the performance of lake plant environmental DNA metabarcoding." (2020) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/108406">https://hdl.handle.net/1911/108406</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/108406en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectenvironmental DNAen_US
dc.subjecteDNAen_US
dc.subjectbiodiversityen_US
dc.subjectconservationen_US
dc.subjectmetabarcodingen_US
dc.subjectfreshwateren_US
dc.subjectaquatic plantsen_US
dc.titleThe role of sampling location and depth in the performance of lake plant environmental DNA metabarcodingen_US
dc.typeThesisen_US
dc.type.genrecomputer languages and codeen_US
dc.type.materialTexten_US
thesis.degree.departmentEcology and Evolutionary Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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