Conformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transfer

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dc.contributor.advisorLandes, Christy F
dc.contributor.committeeMemberClementi, Cecilia
dc.contributor.committeeMemberPhillips, George
dc.creatorCooper, David
dc.date.accessioned2016-01-07T17:26:23Z
dc.date.available2016-01-07T17:26:23Z
dc.date.created2015-05
dc.date.issued2015-04-21
dc.date.submittedMay 2015
dc.date.updated2016-01-07T17:26:23Z
dc.description.abstractGlutamate receptors perform a critical role in the nervous system, mediating synaptic transmission through cellular membranes. These proteins have developed a quick response to agonist presence and, when activated, undergo a conformational shift that allows calcium ion transport across the membrane. In a number of neurological diseases, genetic variants can cause mutations that disrupt the balance between fast binding response and desensitization resulting in over- or under- signaling in affected cells. Herein it describes the use of single molecule Förster resonance energy transfer to observe the bound form of the glutamate receptors and subsequent dynamics. For a complete understanding of the dynamics involved in the conformational landscape, the response of the receptor to a variety of known full and partial agonists is tested. Additionally multiple receptor types are investigated to determine if the conformational pathway is conserved between the different types of glutamate receptors. The results from this project allows for a more complete understanding of the relationship between conformation and functionality of glutamate receptors.
dc.format.mimetypeapplication/pdf
dc.identifier.citationCooper, David. "Conformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transfer." (2015) Diss., Rice University. <a href="https://hdl.handle.net/1911/87759">https://hdl.handle.net/1911/87759</a>.
dc.identifier.urihttps://hdl.handle.net/1911/87759
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectglutamate receptors
dc.subjectsingle molecule
dc.subjectFRET
dc.subject
dc.titleConformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transfer
dc.typeThesis
dc.type.materialText
thesis.degree.departmentChemistry
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
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