Conformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transfer

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dc.contributor.advisorLandes, Christy Fen_US
dc.contributor.committeeMemberClementi, Ceciliaen_US
dc.contributor.committeeMemberPhillips, Georgeen_US
dc.creatorCooper, Daviden_US
dc.date.accessioned2016-01-07T17:26:23Zen_US
dc.date.available2016-01-07T17:26:23Zen_US
dc.date.created2015-05en_US
dc.date.issued2015-04-21en_US
dc.date.submittedMay 2015en_US
dc.date.updated2016-01-07T17:26:23Zen_US
dc.description.abstractGlutamate receptors perform a critical role in the nervous system, mediating synaptic transmission through cellular membranes. These proteins have developed a quick response to agonist presence and, when activated, undergo a conformational shift that allows calcium ion transport across the membrane. In a number of neurological diseases, genetic variants can cause mutations that disrupt the balance between fast binding response and desensitization resulting in over- or under- signaling in affected cells. Herein it describes the use of single molecule Förster resonance energy transfer to observe the bound form of the glutamate receptors and subsequent dynamics. For a complete understanding of the dynamics involved in the conformational landscape, the response of the receptor to a variety of known full and partial agonists is tested. Additionally multiple receptor types are investigated to determine if the conformational pathway is conserved between the different types of glutamate receptors. The results from this project allows for a more complete understanding of the relationship between conformation and functionality of glutamate receptors.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationCooper, David. "Conformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transfer." (2015) Diss., Rice University. <a href="https://hdl.handle.net/1911/87759">https://hdl.handle.net/1911/87759</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/87759en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectglutamate receptorsen_US
dc.subjectsingle moleculeen_US
dc.subjectFRETen_US
dc.subjecten_US
dc.titleConformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transferen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemistryen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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