First evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development

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dc.contributor.advisorGomer, Richard H.
dc.creatorSchwartz, Jeanette Marie
dc.date.accessioned2009-06-04T08:46:56Z
dc.date.available2009-06-04T08:46:56Z
dc.date.issued1996
dc.description.abstractIndirect immunofluorescence in conjunction with Northern and Western blot analysis were used to identify the presence of skelemin, a myosin-associated protein, in smooth muscle. Feline uterus was cryosectioned and triple-stained for skelemin, smooth muscle myosin, and desmin. I observed that skelemin antibodies colocalized with the myosin-II filaments as well as the desmin intermediate filament cytoskeleton. This is the first evidence of a myosin-associated protein within smooth muscle, and it raises the possibility that smooth muscle myosin may be more organized than has been assumed. Antisense oligonucleotide treatment was used to analyze the underexpression of skelemin protein in developing embryoid bodies. Skelemin underexpression was seen to cause loss of cell-cell as well as cell-substrate adhesion. Time lapse video microscopy was used to analyze the role of the cell cycle in cell type choice during mammalian development. Discussed are methods employed to improve resolution for accurate analysis.
dc.format.extent63 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS BIOCHEM. 1997 SCHWARTZ
dc.identifier.citationSchwartz, Jeanette Marie. "First evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development." (1996) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/17130">https://hdl.handle.net/1911/17130</a>.
dc.identifier.urihttps://hdl.handle.net/1911/17130
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectMolecular biology
dc.subjectCell biology
dc.titleFirst evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBiochemistry and Cell Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts
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