Browsing by Author "Zhang, Jianhua"

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    Decoupling Lineage-Associated Genes in Acute Myeloid Leukemia Reveals Inflammatory and Metabolic Signatures Associated With Outcomes
    (Frontiers, 2021) Abbas, Hussein A.; Mohanty, Vakul; Wang, Ruiping; Huang, Yuefan; Liang, Shaoheng; Wang, Feng; Zhang, Jianhua; Qiu, Yihua; Hu, Chenyue W.; Qutub, Amina A.; Dail, Monique; Bolen, Christopher R.; Daver, Naval; Konopleva, Marina; Futreal, Andrew; Chen, Ken; Wang, Linghua; Kornblau, Steven M.
    Acute myeloid leukemia (AML) is a heterogeneous disease with variable responses to therapy. Cytogenetic and genomic features are used to classify AML patients into prognostic and treatment groups. However, these molecular characteristics harbor significant patient-to-patient variability and do not fully account for AML heterogeneity. RNA-based classifications have also been applied in AML as an alternative approach, but transcriptomic grouping is strongly associated with AML morphologic lineages. We used a training cohort of newly diagnosed AML patients and conducted unsupervised RNA-based classification after excluding lineage-associated genes. We identified three AML patient groups that have distinct biological pathways associated with outcomes. Enrichment of inflammatory pathways and downregulation of HOX pathways were associated with improved outcomes, and this was validated in 2 independent cohorts. We also identified a group of AML patients who harbored high metabolic and mTOR pathway activity, and this was associated with worse clinical outcomes. Using a comprehensive reverse phase protein array, we identified higher mTOR protein expression in the highly metabolic group. We also identified a positive correlation between degree of resistance to venetoclax and mTOR activation in myeloid and lymphoid cell lines. Our approach of integrating RNA, protein, and genomic data uncovered lineage-independent AML patient groups that share biologic mechanisms and can inform outcomes independent of commonly used clinical and demographic variables; these groups could be used to guide therapeutic strategies.
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    Full-color fluorescent carbon quantum dots
    (AAAS, 2020) Wang, Liang; Li, Weitao; Yin, Luqiao; Liu, Yijian; Guo, Huazhang; Lai, Jiawei; Han, Yu; Li, Gao; Li, Ming; Zhang, Jianhua; Vajtai, Robert; Ajayan, Pulickel M.; Wu, Minghong
    Quantum dots have innate advantages as the key component of optoelectronic devices. For white light–emitting diodes (WLEDs), the modulation of the spectrum and color of the device often involves various quantum dots of different emission wavelengths. Here, we fabricate a series of carbon quantum dots (CQDs) through a scalable acid reagent engineering strategy. The growing electron-withdrawing groups on the surface of CQDs that originated from acid reagents boost their photoluminescence wavelength red shift and raise their particle sizes, elucidating the quantum size effect. These CQDs emit bright and remarkably stable full-color fluorescence ranging from blue to red light and even white light. Full-color emissive polymer films and all types of high–color rendering index WLEDs are synthesized by mixing multiple kinds of CQDs in appropriate ratios. The universal electron-donating/withdrawing group engineering approach for synthesizing tunable emissive CQDs will facilitate the progress of carbon-based luminescent materials for manufacturing forward-looking films and devices.