Browsing by Author "Yang, Zheng"
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Item Repair of Osteochondral Defects with Rehydrated Freeze-Dried Oligo[Poly(Ethylene Glycol) Fumarate] Hydrogels Seeded with Bone Marrow Mesenchymal Stem Cells in a Porcine Model(Mary Ann Liebert, Inc., 2013) Lim, Chin Tat; Ren, Xiafei; Afizah, Mohd Hassan; Tarigan-Panjaitan, Sari; Yang, Zheng; Wu, Yingnan; Chian, Kerm Sin; Mikos, Antonios G.; Hui, James Hoi Po; BioScience Research CollaborativeCurrent surgical techniques for osteochondral injuries in young active patients are inadequate clinically. Novel strategies in tissue engineering are continuously explored in this area. Despite numerous animal studies that have shown encouraging results, very few large-scale clinical trials have been done to address this area of interest. To facilitate the eventual translation from rabbit to human subjects, we have performed a study using bone marrowderived mesenchymal stem cell (BMSC)ヨoligo[poly(ethylene glycol) fumarate] (OPF) hydrogel scaffold in a porcine model. Our objective was to analyze the morphology of BMSCs seeded into rehydrated freeze-dried OPF hydrogel and in vivo gross morphological and histological outcome of defects implanted with the BMSCs-OPF scaffold in a porcine model. The analyses were based on magnified histologic sections for different types of cartilage repair tissues, the outcome of the subchondral bone, scaffold, and statistical assessment of neotissue-filling percentage, cartilage phenotype, and Wakitani scores. The morphology of the BMSCs seeded into the rehydrated freeze-dried OPF scaffold was observed 24 h after cell seeding, through the phase-contrast microscope. The three-dimensional and cross-sectional structure of the fabrication was analyzed through confocal microscopy and histological methods, respectively. The BMSCs remained viable and were condensed into many pellet-like cell masses with a diameter ranging from 28.5 to 298.4 (113.5 - 47.9) mm in the OPF scaffold. In vivo osteochondral defect repair was tested in 12 defects created in six 8-month-old Prestige World Genetics micropigs. The implantation of scaffold alone was used for control. Gross morphological, histological, and statistical analyses were performed at 4 months postoperatively. The scaffoldヨMSC treatment led to 99% defect filling, with 84% hyaline-like cartilage at 4 months, which was significantly ( p < 0.0001) more than the 54% neotissue filling and 39% hyaline-like cartilage obtained in the scaffold-only group. The implantation of BMSCs in freeze-dried OPF hydrogel scaffold, which created a conducive environment for cell infiltration and clustering, could fully repair chondral defects with hyaline-like cartilage. This protocol provides a clinically feasible procedure for osteochondral defect treatment.Item Viscosity Evaluation of Heavy Oils from NMR Well Logging(2011) Yang, Zheng; Hirasaki, George J.Heavy oil is characterized by its high viscosity, which is a major obstacle to both logging and recovery. Due to the loss of T 2 information shorter than the echo spacing ( TE ), estimation of heavy oil properties from NMR T 2 measurements is usually problematic. In this work, a new method has been developed to overcome the echo spacing restriction of NMR spectrometer during the measurement of heavy oil. A FID measurement supplemented the CPMG in an effort to recover the lost T 2 data. Constrained by the initial magnetization ( M 0 ) estimated from the FID and Curie's law and assuming lognormal distribution for bitumen, the corrected T 2 of bitumen can be obtained. This new method successfully overcomes the TE restriction of the NMR spectrometer and is nearly independent on the TE applied in the measurement. This method was applied in the measurement of systems at elevated temperatures (8 ∼ 90 °C) and some important petrophysical properties of Athabasca bitumen, such as hydrogen index ( HI ), fluid content and viscosity were evaluated by using the corrected T 2 . Well log NMR T 2 measurements of bitumen appear to be significantly longer than the laboratory results. This is likely due to the dissolved gas in bitumen. The T 2 distribution depends on oil viscosity and dissolved gas concentration, which can vary throughout the field. In this work, the viscosity and laboratory NMR measurements were made on the recombined live bitumen sample and the synthetic Brookfield oil as a function of dissolved gas concentrations. The effects of CH 4 , CO 2 , and C 2 H 6 on the viscosity and T 2 response of these two heavy oils at different saturation pressures were investigated. The investigations on live oil viscosity show that, regardless of the gas type used for saturation, the live oil T 2 correlates with viscosity/temperature ratio on a log-log scale. More importantly, the changes of T 2 and viscosity/temperature ratio caused by solution gas follows the same trend of those caused by temperature variations on the dead oil. This conclusion holds for both the bitumen and the synthetic Brookfield oil. This finding on the relationship between the oil T 2 and its corresponding viscosity/temperature ratio creates a way for in-situ viscosity evaluation of heavy oil through NMR well logging.