Browsing by Author "Rosales, Stephanie M."

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    Building consensus around the assessment and interpretation of Symbiodiniaceae diversity
    (PeerJ, Inc, 2023) Davies, Sarah W.; Gamache, Matthew H.; Howe-Kerr, Lauren I.; Kriefall, Nicola G.; Baker, Andrew C.; Banaszak, Anastazia T.; Bay, Line Kolind; Bellantuono, Anthony J.; Bhattacharya, Debashish; Chan, Cheong Xin; Claar, Danielle C.; Coffroth, Mary Alice; Cunning, Ross; Davy, Simon K.; Campo, Javier del; Díaz-Almeyda, Erika M.; Frommlet, Jörg C.; Fuess, Lauren E.; González-Pech, Raúl A.; Goulet, Tamar L.; Hoadley, Kenneth D.; Howells, Emily J.; Hume, Benjamin C. C.; Kemp, Dustin W.; Kenkel, Carly D.; Kitchen, Sheila A.; LaJeunesse, Todd C.; Lin, Senjie; McIlroy, Shelby E.; McMinds, Ryan; Nitschke, Matthew R.; Oakley, Clinton A.; Peixoto, Raquel S.; Prada, Carlos; Putnam, Hollie M.; Quigley, Kate; Reich, Hannah G.; Reimer, James Davis; Rodriguez-Lanetty, Mauricio; Rosales, Stephanie M.; Saad, Osama S.; Sampayo, Eugenia M.; Santos, Scott R.; Shoguchi, Eiichi; Smith, Edward G.; Stat, Michael; Stephens, Timothy G.; Strader, Marie E.; Suggett, David J.; Swain, Timothy D.; Tran, Cawa; Traylor-Knowles, Nikki; Voolstra, Christian R.; Warner, Mark E.; Weis, Virginia M.; Wright, Rachel M.; Xiang, Tingting; Yamashita, Hiroshi; Ziegler, Maren; Correa, Adrienne M. S.; Parkinson, John Everett
    Within microeukaryotes, genetic variation and functional variation sometimes accumulate more quickly than morphological differences. To understand the evolutionary history and ecology of such lineages, it is key to examine diversity at multiple levels of organization. In the dinoflagellate family Symbiodiniaceae, which can form endosymbioses with cnidarians (e.g., corals, octocorals, sea anemones, jellyfish), other marine invertebrates (e.g., sponges, molluscs, flatworms), and protists (e.g., foraminifera), molecular data have been used extensively over the past three decades to describe phenotypes and to make evolutionary and ecological inferences. Despite advances in Symbiodiniaceae genomics, a lack of consensus among researchers with respect to interpreting genetic data has slowed progress in the field and acted as a barrier to reconciling observations. Here, we identify key challenges regarding the assessment and interpretation of Symbiodiniaceae genetic diversity across three levels: species, populations, and communities. We summarize areas of agreement and highlight techniques and approaches that are broadly accepted. In areas where debate remains, we identify unresolved issues and discuss technologies and approaches that can help to fill knowledge gaps related to genetic and phenotypic diversity. We also discuss ways to stimulate progress, in particular by fostering a more inclusive and collaborative research community. We hope that this perspective will inspire and accelerate coral reef science by serving as a resource to those designing experiments, publishing research, and applying for funding related to Symbiodiniaceae and their symbiotic partnerships.
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    Coral Bleaching Phenotypes Associated With Differential Abundances of Nucleocytoplasmic Large DNA Viruses
    (Frontiers, 2020) Messyasz, Adriana; Rosales, Stephanie M.; Mueller, Ryan S.; Sawyer, Teresa; Correa, Adrienne M.S.; Thurber, Andrew R.; Thurber, Rebecca Vega
    Eukaryotic viruses and bacteriophage have been implicated in disease and bleaching in corals, but the compositional and functional diversity of these viruses in healthy and compromised hosts remains underexplored. To investigate whether viral assemblages differ in concert with coral bleaching, we collected bleached and non-bleached conspecific pairs of corals during a minor bleaching event in 2016 from reefs on the island of Mo’orea, French Polynesia. Using electron microscopy (EM), we identified several viral particle types, all reminiscent of medium to large-sized nucleocytoplasmic large DNA viruses (NCLDV). We also found that viral metagenomes from bleached corals have significantly more eukaryotic virus sequences, whereas bacteriophage sequences are significantly more abundant in viral metagenomes from non-bleached colonies. In this study, we also initiated the assembly of the first eukaryotic dsDNA coral virus genome. Based on our EM imagery and our taxonomic annotations of viral metagenome sequences, we hypothesize that this genome represents a novel, phylogenetically distinct member of the NCLDVs, with its closest sequenced relative being a distant marine flagellate-associated virus. We also showed that this NCLDV is abundant in bleached corals, but it is also present in apparently healthy corals, suggesting it plays a role in the onset and/or severity of coral bleaching.