Browsing by Author "Poddar, Nitesh"
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Item Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems(John Wiley & Sons, Inc., 2013) Cooper, David; Uhm, Heui; Tauzin, Lawrence J.; Poddar, Nitesh; Landes, Christy F.We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes