Browsing by Author "Nguyen, Thuc-Uyen"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Development of Hyperspectral Imagers for Snapshot Optical Coherence Tomography
    (2014-04-25) Nguyen, Thuc-Uyen; Tkaczyk, Tomasz S.; Richards-Kortum, Rebecca Rae; Baraniuk, Richard G.; Pierce, Mark C
    Optical Coherence Tomography (OCT) is an established interferometry-based technique for volumetric tissue imaging with micrometer resolution, best known in many medical applications such as ophthalmologic imaging and endoscopy. Several clinically recognized examples include retinal imaging to detect glaucoma and age-related macular degeneration (AMD) or cardiovascular imaging when employed with a catheter. Scanning mechanism presenting in all current OCT technology requires moving parts, often limiting the system’s compactness, compromising light throughput and risking unwanted movement. Snapshot imaging thus allows fast and high-throughput acquisition while minimizing motion artifacts caused by instrumental vibration or samples’ transient nature. This thesis presents novel work contributing to the development of a snapshot 3-Dimensional OCT (3D-OCT) system. With theoretical and experimental evaluations, different hyperspectral imaging designs were surveyed to provide enhancements such as high throughput, dense spectral sampling, high sensitivity toward the appropriate spectrum and spatial-spectral tunability. A proof-of-concept snapshot 3D-OCT system is introduced to simultaneously collect signals of a volumetric datacube, enabling cellular visualization of scattering biological samples. This system affords diffraction-limited performance with reduced motion and requires minimal computational time.
  • Thumbnail Image
    Item
    High performance image mapping spectrometer (IMS) for snapshot hyperspectral imaging applications
    (Optical Society of America, 2019) Pawlowski, Michal E.; Dwight, Jason G.; Nguyen, Thuc-Uyen; Tkaczyk, Tomasz S.
    A high performance, snapshot Image Mapping Spectrometer was developed that provides fast image acquisition (100 Hz) of 16 bit hyperspectral data cubes (210x210x46) over a spectral range of 515-842 nm. Essential details of the opto-mechanical design are presented. Spectral accuracy, precision, and image reconstruction metrics such as resolution are discussed. Fluorescently stained cell samples were used to directly compare the data obtained using newly developed and the reference image mapping spectrometer. Additional experimental results are provided to demonstrate the abilities of the new spectrometer to acquire highly-resolved, motion-artifact-free hyperspectral images at high temporal sampling rates.
  • Thumbnail Image
    Item
    Snapshot 3D optical coherence tomography system using image mapping spectrometry
    (Optical Society of America, 2013) Nguyen, Thuc-Uyen; Pierce, Mark C.; Higgins, Laura; Tkaczyk, Tomasz S.
    A snapshot 3-Dimensional Optical Coherence Tomography system was developed using Image Mapping Spectrometry. This system can give depth information (Z) at different spatial positions (XY) within one camera integration time to potentially reduce motion artifact and enhance throughput. The current (x,y,λ) datacube of (85×356×117) provides a 3D visualization of sample with 400 μm depth and 13.4 μm in transverse resolution. Axial resolution of 16.0 μm can also be achieved in this proof-of-concept system. We present an analysis of the theoretical constraints which will guide development of future systems with increased imaging depth and improved axial and lateral resolutions.