Browsing by Author "Harris, Anthony D."

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    Role of miR-2392 in driving SARS-CoV-2 infection
    (Elsevier, 2021) McDonald, J. Tyson; Enguita, Francisco J.; Taylor, Deanne; Griffin, Robert J.; Priebe, Waldemar; Emmett, Mark R.; Sajadi, Mohammad M.; Harris, Anthony D.; Clement, Jean; Dybas, Joseph M.; Aykin-Burns, Nukhet; Guarnieri, Joseph W.; Singh, Larry N.; Grabham, Peter; Baylin, Stephen B.; Yousey, Aliza; Pearson, Andrea N.; Corry, Peter M.; Saravia-Butler, Amanda; Aunins, Thomas R.; Sharma, Sadhana; Nagpal, Prashant; Meydan, Cem; Foox, Jonathan; Mozsary, Christopher; Cerqueira, Bianca; Zaksas, Viktorija; Singh, Urminder; Wurtele, Eve Syrkin; Costes, Sylvain V.; Davanzo, Gustavo Gastão; Galeano, Diego; Paccanaro, Alberto; Meinig, Suzanne L.; Hagan, Robert S.; Bowman, Natalie M.; Wallet, Shannon M.; Maile, Robert; Wolfgang, Matthew C.; Hagan, Robert S.; Mock, Jason R.; Bowman, Natalie M.; Torres-Castillo, Jose L.; Love, Miriya K.; Meinig, Suzanne L.; Lovell, Will; Rice, Colleen; Mitchem, Olivia; Burgess, Dominique; Suggs, Jessica; Jacobs, Jordan; Wolfgang, Matthew C.; Altinok, Selin; Sapoval, Nicolae; Treangen, Todd J.; Moraes-Vieira, Pedro M.; Vanderburg, Charles; Wallace, Douglas C.; Schisler, Jonathan C.; Mason, Christopher E.; Chatterjee, Anushree; Meller, Robert; Beheshti, Afshin
    MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation that have a major impact on many diseases and provide an exciting avenue toward antiviral therapeutics. From patient transcriptomic data, we determined that a circulating miRNA, miR-2392, is directly involved with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) machinery during host infection. Specifically, we show that miR-2392 is key in driving downstream suppression of mitochondrial gene expression, increasing inflammation, glycolysis, and hypoxia, as well as promoting many symptoms associated with coronavirus disease 2019 (COVID-19) infection. We demonstrate that miR-2392 is present in the blood and urine of patients positive for COVID-19 but is not present in patients negative for COVID-19. These findings indicate the potential for developing a minimally invasive COVID-19 detection method. Lastly, using in vitro human and in vivo hamster models, we design a miRNA-based antiviral therapeutic that targets miR-2392, significantly reduces SARS-CoV-2 viability in hamsters, and may potentially inhibit a COVID-19 disease state in humans.