Browsing by Author "Genin, Guy M."

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Measurement of mechanical tractions exerted by cells within three-dimensional matrices
    (Nature Publishing Group, 2010) Legant, Wesley R.; Miller, Jordan S.; Blakely, Brandon L.; Cohen, Daniel M.; Genin, Guy M.; Chen, Christopher S.; Bioengineering
    Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated within well-defined elastic hydrogel matrices. We apply this approach to measure tractions from a variety of cell types and contexts, and reveal patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix.