Browsing by Author "Davies, Sarah W."

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    Building consensus around the assessment and interpretation of Symbiodiniaceae diversity
    (PeerJ, Inc, 2023) Davies, Sarah W.; Gamache, Matthew H.; Howe-Kerr, Lauren I.; Kriefall, Nicola G.; Baker, Andrew C.; Banaszak, Anastazia T.; Bay, Line Kolind; Bellantuono, Anthony J.; Bhattacharya, Debashish; Chan, Cheong Xin; Claar, Danielle C.; Coffroth, Mary Alice; Cunning, Ross; Davy, Simon K.; Campo, Javier del; Díaz-Almeyda, Erika M.; Frommlet, Jörg C.; Fuess, Lauren E.; González-Pech, Raúl A.; Goulet, Tamar L.; Hoadley, Kenneth D.; Howells, Emily J.; Hume, Benjamin C. C.; Kemp, Dustin W.; Kenkel, Carly D.; Kitchen, Sheila A.; LaJeunesse, Todd C.; Lin, Senjie; McIlroy, Shelby E.; McMinds, Ryan; Nitschke, Matthew R.; Oakley, Clinton A.; Peixoto, Raquel S.; Prada, Carlos; Putnam, Hollie M.; Quigley, Kate; Reich, Hannah G.; Reimer, James Davis; Rodriguez-Lanetty, Mauricio; Rosales, Stephanie M.; Saad, Osama S.; Sampayo, Eugenia M.; Santos, Scott R.; Shoguchi, Eiichi; Smith, Edward G.; Stat, Michael; Stephens, Timothy G.; Strader, Marie E.; Suggett, David J.; Swain, Timothy D.; Tran, Cawa; Traylor-Knowles, Nikki; Voolstra, Christian R.; Warner, Mark E.; Weis, Virginia M.; Wright, Rachel M.; Xiang, Tingting; Yamashita, Hiroshi; Ziegler, Maren; Correa, Adrienne M. S.; Parkinson, John Everett
    Within microeukaryotes, genetic variation and functional variation sometimes accumulate more quickly than morphological differences. To understand the evolutionary history and ecology of such lineages, it is key to examine diversity at multiple levels of organization. In the dinoflagellate family Symbiodiniaceae, which can form endosymbioses with cnidarians (e.g., corals, octocorals, sea anemones, jellyfish), other marine invertebrates (e.g., sponges, molluscs, flatworms), and protists (e.g., foraminifera), molecular data have been used extensively over the past three decades to describe phenotypes and to make evolutionary and ecological inferences. Despite advances in Symbiodiniaceae genomics, a lack of consensus among researchers with respect to interpreting genetic data has slowed progress in the field and acted as a barrier to reconciling observations. Here, we identify key challenges regarding the assessment and interpretation of Symbiodiniaceae genetic diversity across three levels: species, populations, and communities. We summarize areas of agreement and highlight techniques and approaches that are broadly accepted. In areas where debate remains, we identify unresolved issues and discuss technologies and approaches that can help to fill knowledge gaps related to genetic and phenotypic diversity. We also discuss ways to stimulate progress, in particular by fostering a more inclusive and collaborative research community. We hope that this perspective will inspire and accelerate coral reef science by serving as a resource to those designing experiments, publishing research, and applying for funding related to Symbiodiniaceae and their symbiotic partnerships.
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    Gene Expression of Endangered Coral (Orbicella spp.) in Flower Garden Banks National Marine Sanctuary After Hurricane Harvey
    (Frontiers, 2019) Wright, Rachel M.; Correa, Adrienne M.S.; Quigley, Lucinda A.; Santiago-Vázquez, Lory Z.; Shamberger, Kathryn E.F.; Davies, Sarah W.
    About 190 km south of the Texas–Louisiana border, the East and West Flower Garden Banks (FGB) have maintained > 50% coral cover with infrequent and minor incidents of disease or bleaching since monitoring began in the 1970s. However, a mortality event, affecting 5.6 ha (2.6% of the area) of the East FGB, occurred in late July 2016 and coincided with storm-generated freshwater runoff extending offshore and over the reef system. To capture the immediate effects of storm-driven freshwater runoff on coral and symbiont physiology, we leveraged the heavy rainfall associated with Hurricane Harvey in late August 2017 by sampling FGB corals at two time points: September 2017, when surface water salinity was reduced (∼34 ppt); and 1 month later when salinity had returned to typical levels (∼36 ppt in October 2017). Tissue samples (N = 47) collected midday were immediately preserved for gene expression profiling from two congeneric coral species (Orbicella faveolata and Orbicella franksi) from the East and West FGB to determine the physiological consequences of storm-derived runoff. In the coral, differences between host species and sampling time points accounted for the majority of differentially expressed genes. Gene ontology enrichment for genes differentially expressed immediately after Hurricane Harvey indicated increases in cellular oxidative stress responses. Although tissue loss was not observed on FGB reefs following Hurricane Harvey, our results suggest that poor water quality following this storm caused FGB corals to experience sub-lethal stress. We also found dramatic expression differences across sampling time points in the coral’s algal symbiont, Breviolum minutum. Some of these differentially expressed genes may be involved in the symbionts’ response to changing environments, including a group of differentially expressed post-transcriptional RNA modification genes. In this study, we cannot disentangle the effects of reduced salinity from the collection time point, so these expression patterns could also be related to seasonality. These findings highlight the urgent need for continued monitoring of these reef systems to establish a baseline for gene expression of healthy corals in the FGB system across seasons, as well as the need for integrated solutions to manage stormwater runoff in the Gulf of Mexico.
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    On a Reef Far, Far Away: Anthropogenic Impacts Following Extreme Storms Affect Sponge Health and Bacterial Communities
    (Frontiers Media S.A., 2021) Shore, Amanda; Sims, Jordan A.; Grimes, Michael; Howe-Kerr, Lauren I.; Grupstra, Carsten G.B.; Doyle, Shawn M.; Stadler, Lauren B.; Sylvan, Jason B.; Shamberger, Kathryn E.F.; Davies, Sarah W.; Santiago-Vázquez, Lory Z.; Correa, Adrienne M.S.
    Terrestrial runoff can negatively impact marine ecosystems through stressors including excess nutrients, freshwater, sediments, and contaminants. Severe storms, which are increasing with global climate change, generate massive inputs of runoff over short timescales (hours to days); such runoff impacted offshore reefs in the northwest Gulf of Mexico (NW GoM) following severe storms in 2016 and 2017. Several weeks after coastal flooding from these events, NW GoM reef corals, sponges, and other benthic invertebrates ~185 km offshore experienced mortality (2016 only) and/or sub-lethal stress (both years). To assess the impact of storm-derived runoff on reef filter feeders, we characterized the bacterial communities of two sponges, Agelas clathrodes and Xestospongia muta, from offshore reefs during periods of sub-lethal stress and no stress over a three-year period (2016-2018). Sponge-associated and seawater-associated bacterial communities were altered during both flood years. Additionally, we found evidence of wastewater contamination (based on 16S rRNA gene libraries and quantitative PCR) in offshore sponge samples, but not in seawater samples, following these flood years. Signs of wastewater contamination were absent during the no-flood year. We show that flood events from severe storms have the capacity to reach offshore reef ecosystems and impact resident benthic organisms. Such impacts are most readily detected if baseline data on organismal physiology and associated microbiome composition are available. This highlights the need for molecular and microbial time series of benthic organisms in near- and offshore reef ecosystems, and the continued mitigation of stormwater runoff and climate change impacts.