Browsing by Author "Boere, Kristel W.M."

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    Injectable hydrogels
    (2016-03-15) Mikos, Antonios G.; Kasper, Kurtis F.; Ekenseair, Adam K.; Vo, Tiffany N.; Boere, Kristel W.M.; Touchet, Tyler J.; Rice University; United States Patent and Trademark Office
    The present disclosure generally relates to injectable compositions. More particularly, the present disclosure relates to injectable, thermogelling hydrogels and associated methods. In one embodiment, the present disclosure provides for a composition comprising a poly(N-isopropylacrylamide)-based macromer and a polyamidoamine-based macromer.
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    Structure-Property Evaluation of Thermally and Chemically Gelling Injectable Hydrogels for Tissue Engineering
    (American Chemical Society, 2012) Ekenseair, Adam K.; Boere, Kristel W.M.; Tzouanas, Stephanie N.; Vo, Tiffany N.; Kasper, F. Kurtis; Mikos, Antonios G.; Bioengineering
    The impact of synthesis and solution formulation parameters on the swelling and mechanical properties of a novel class of thermally and chemically gelling hydrogels combining poly(N-isopropylacrylamide)-based thermogelling macromers containing pendant epoxy rings with polyamidoamine-based hydrophilic and degradable diamine cross-linking macromers was evaluated. Through variation of network hydrophilicity and capacity for chain rearrangement, the often problematic tendency of thermogelling hydrogels to undergo significant syneresis was addressed. The demonstrated ability to tune postformation dimensional stability easily at both the synthesis and formulation stages represents a significant novel contribution toward efforts to utilize poly(N-isopropylacrylamide)-based polymers as injectable biomaterials. Furthermore, the cytocompatibility of the hydrogel system under relevant conditions was established while demonstrating time- and dose-dependent cytotoxicity at high solution osmolality. Such injectable in situ forming degradable hydrogels with tunable water content are promising candidates for many tissue-engineering applications, particularly for cell delivery to promote rapid tissue regeneration in non-load-bearing defects.
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    Synthesis and Characterization of Thermally and Chemically Gelling Injectable Hydrogels for Tissue Engineering
    (American Chemical Society, 2012) Ekenseair, Adam K.; Boere, Kristel W.M.; Tzouanas, Stephanie N.; Vo, Tiffany N.; Kasper, F. Kurtis; Mikos, Antonios G.; Bioengineering
    Novel, injectable hydrogels were developed that solidify through a dual-gelation, physical and chemical, mechanism upon preparation and elevation of temperature to 37°C. A thermogelling, poly(N-isopropylacrylamide)-based macromer with pendant epoxy rings and a hydrolyticallydegradable polyamidoamine-based diamine crosslinker were synthesized, characterized, and combined to produce in situ forming hydrogel constructs. Network formation through the epoxyamine reaction was shown to be rapid and facile, and the progressive incorporation of the hydrophilic polyamidoamine crosslinker into the hydrogel was shown to mitigate the often problematic tendency of thermogelling materials to undergo significant post-formation gel syneresis. The results suggest that this novel class of injectable hydrogels may be attractive substrates for tissue engineering applications due to the synthetic versatility of the component materials and beneficial hydrogel gelation kinetics and stability.