Browsing by Author "Bhardwaj, M."
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Item Structural characterization of DynU16, a START/Bet v1-like protein involved in dynemicin biosynthesis(International Union of Crystallography, 2021) Alvarado, S. K.; Miller, M. D.; Bhardwaj, M.; Thorson, J. S.; Van Lanen, S. G.; Phillips, G. N.The 1.5 Å resolution crystal structure of DynU16, a protein identified in the dynemicin-biosynthetic gene cluster, is reported. The structure adopts a di-domain helix-grip fold with a uniquely positioned open cavity connecting the domains. The elongated dimensions of the cavity appear to be compatible with the geometry of a linear polyene, suggesting the involvement of DynU16 in the upstream steps of dynemicin biosynthesis.Item The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina(International Union of Crystallography, 2022) Kosgei, A.J.; Miller, M.D.; Bhardwaj, M.; Xu, W.; Thorson, J.S.; Van Lanen, S.G.; Phillips, G.N.Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.