Rice Graduate Student Collection

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https://hdl.handle.net/1911/64826

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Browsing Rice Graduate Student Collection by Author "Bioengineering"

Now showing 1 - 9 of 9
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    Demonstration of Piecewise Cubic Polynomial Fitting on Mesoscale Tester Data
    (Rice University, 2020) Mehta, Shail Maharshi; De Santos, Diego Ricardo; Sridhar, Shweta; Aguayo, Veronica Cristina; Meraz, Carlos Alberto; Mikos, Mary; Grande-Allen, K. Jane; Bioengineering
    Animation of piecewise cubic polynomial fitting to data from a replicate of 1:15 PDMS performed in order to obtain stress values at exact increments of strain
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    Morphological diversity of extracellular vesicles revealed by cryo-electron microscopy
    (ASEMV/AAEV, 10/1/2022) Kapoor, Kshipra S.; McAndrews, Kathleen M.; Biswal, Lisa S.; Kalluri, Raghu; Bioengineering
    Introduction: Exosomes are extracellular vesicles 80-150 nm in diameter, containing proteins, mRNAs, microRNAs, and lipids reflecting the parent cell. While there has been an extensive characterization of the cargo incorporated in exosomes, a detailed morphological analysis of exosomes purified by various isolation techniques has not been performed. Objective: We aimed to determine the heterogeneity of exosomes morphology and if such morphological features are conserved across sample types. Methods: Our study used Cryogenic Electron Microscopy (Cryo-EM) to examine exosome size and morphology. Results: Our results revealed significant diversity in extracellular vesicle morphology independent of the isolation method, suggesting that morphological subpopulations of these vesicles exist. Based on their shape, our analysis classified exosomes into seven categories. In addition, we developed a semi-automatic image analysis framework to accurately characterize exosome attributes and distribution to facilitate reliable quantification of specific bio-nanoparticle features in Cryo-EM micrographs. Conclusions: Morphological features of exosomes inform their biophysical properties, which influence both biodistribution and biological activity in vivo. Our data demonstrating the innate morphological diversity of exosomes may have implications for improving the specificity and precision of exosome-delivered therapeutics. Conflict of interest: R.K. and MD Anderson Cancer Center hold patents in exosome biology and are stock equity holders in Codiak Biosciences Inc. R.K. is a consultant and a scientific advisor of Codiak Biosciences Inc.
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    Parts List, Prices, and Links for Commercial Components of Mesoscale Testing Device
    (Rice University, 2020) Mehta, Shail Maharshi; De Santos, Diego Ricardo; Sridhar, Shweta; Aguayo, Veronica Cristina; Meraz, Carlos Alberto; Mikos, Mary; Grande-Allen, K. Jane; Bioengineering
    File displaying names, prices, quantities, and purchasing links of commercial components of mesoscale testing device
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    Pixel Selection Demonstration Video of 1:10 PDMS from Mesoscale Testing Device on Fluorescence Microscope
    (Rice University, 2020) Mehta, Shail Maharshi; De Santos, Diego Ricardo; Sridhar, Shweta; Aguayo, Veronica Cristina; Meraz, Carlos Alberto; Mikos, Mary; Grande-Allen, K. Jane; Bioengineering
    Testing video taken using a fluorescence microscope at 10x magnification of 1:10 PDMS demonstrating which pixels are selected as belonging to edges to be tracked in yellow. Only every other pixel was checked for the appropriate color to reduce processing time
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    Pixel Selection Demonstration Video of 1:15 PDMS from Mesoscale Testing Device
    (Rice University, 2020) Mehta, Shail Maharshi; De Santos, Diego Ricardo; Sridhar, Shweta; Aguayo, Veronica Cristina; Meraz, Carlos Alberto; Mikos, Mary; Grande-Allen, K. Jane; Bioengineering
    Testing video taken using a stereomicroscope at 0.63x magnification of 1:15 PDMS demonstrating which pixels are selected as belonging to points to be tracked in yellow. Only every other pixel was checked for the appropriate color to reduce processing time
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    Print and CAD Files for Mesoscale Testing Device
    (Rice University, 2020) Mehta, Shail Maharshi; De Santos, Diego Ricardo; Sridhar, Shweta; Aguayo, Veronica Cristina; Meraz, Carlos Alberto; Mikos, Mary; Grande-Allen, K. Jane; Bioengineering
    Folder containing 3D-printable components of mesoscale testing device
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    Simple ultraviolet microscope using off-the-shelf components for point-of-care diagnostics.
    (Rice University, 2/12/2019) Wong, Cindy; Bioengineering
    At the primary care setting, where there are often no or minimal laboratories, examinations often consist of self-testing and rapid diagnostics. Because of this, medical devices must be simple, robust, and easy to operate. To address these concerns, an alternate fluorescence microscope design uses ultraviolet (UV) excitation, since fluorescent dyes that are excitable in the visible region are also excitable by UV. This may allow for the removal of typical excitation, emission, and dichroic filters as optical components absorb UV wavelengths and UV is not detected by silicon based detectors. Additionally, UV has a very low penetration into samples, which may allow for controlling the depth of excitation, and thus the imaging volume. Based on these ideas, we developed a simple fluorescence microscope built completely from off-the-shelf components that uses UV to image fluorescently stained samples. The simple opto-mechanical design of the system may allow it to be more compact and easy to use, as well as decrease the overall cost of the diagnostic device. For biological validation, we imaged whole blood stained with acridine orange and performed a two-part white blood cell differential count.
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    Tracking Demonstration Video of 1:10 PDMS from Mesoscale Testing Device on Fluorescence Microscope
    (Rice University, 2020) Mehta, Shail Maharshi; De Santos, Diego Ricardo; Sridhar, Shweta; Aguayo, Veronica Cristina; Meraz, Carlos Alberto; Mikos, Mary; Grande-Allen, K. Jane; Bioengineering
    Edge tracking output from testing video taken using a fluorescence microscope at 10x magnification of 1:15 PDMS. Yellow lines indicate tracking paths
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    Tracking Demonstration Video of 1:15 PDMS from Mesoscale Testing Device
    (Rice University, 2020) Mehta, Shail Maharshi; De Santos, Diego Ricardo; Sridhar, Shweta; Aguayo, Veronica Cristina; Meraz, Carlos Alberto; Mikos, Mary; Grande-Allen, K. Jane; Bioengineering
    Point tracking output from testing video taken using a stereomicroscope at 0.63x magnification of 1:15 PDMS. Yellow lines indicate tracking paths. One red line directly connects the center points of the sample-attached and static element points. The other red line indicates where the sample-attached point would have been without the presence of a sample. The difference between the endpoints of these lines is the deflection, shown using blue lines