Drezek, Rebekah A.2013-09-162013-09-162013-09-162013-09-162013-052013-09-16May 2013Noelck, Sterling. "Evaluating the Effects of Cell Sample Preparation on FTIR Cancer Detection." (2013) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/72015">https://hdl.handle.net/1911/72015</a>.https://hdl.handle.net/1911/72015This thesis examines some of the challenges involved with using FTIR spectroscopy for cancer detection including sample preparation and correcting for distortion from cell scattering. Sample preparation affects the spectra differently depending on the cell type, and can lead to significant changes in cancer biomarkers for a given cell type. Biomarkers derived from specific cancer types under one sample preparation are not reliable for other cancer types, and may not be suitable for the same cancer type using a different sample preparation. Cell scattering can also significantly affect the cell spectra, and as a result, correcting for the cell scattering distortion leads to changes in the biomarkers. For reliable cancer detection controlling variability is critical, especially in the complex spectra of biological samples. Standard sample preparation methods and scattering correction post-processing could improve comparison of cancer detection methods.application/pdfengCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.FTIRSample preparationCancer cellsCancer detectionBiophotonicsThesis2013-09-16123456789/ETD-2013-05-514