Jiang, XueWilkirson, Elizabeth C.Bailey, Aaron O.Russell, William K.Lillehoj, Peter B.2024-08-222024-08-222024Jiang, X., Wilkirson, E. C., Bailey, A. O., Russell, W. K., & Lillehoj, P. B. (2024). Microneedle-based sampling of dermal interstitial fluid using a vacuum-assisted skin patch. Cell Reports Physical Science, 5(6). https://doi.org/10.1016/j.xcrp.2024.101975https://hdl.handle.net/1911/117686Interstitial fluid (ISF) contains a wealth of biomolecules, yet it is underutilized for diagnostic testing due to a lack of rapid and simple techniques for collecting abundant amounts of fluid. Here, we report a simple and minimally invasive technique for rapidly sampling larger quantities of ISF from human skin. A microneedle array is used to generate micropores in skin from which ISF is extracted using a vacuum-assisted skin patch. Using this technique, an average of 20.8 μL of dermal ISF is collected in 25 min, which is an ∼6-fold improvement over existing sampling methods. Proteomic analysis of collected ISF reveals that it has nearly identical protein composition as blood, and >600 medically relevant biomarkers are identified. Toward this end, we demonstrate the detection of SARS-CoV-2 neutralizing antibodies in ISF collected from COVID-19 vaccinees using two commercial immunoassays, showcasing the utility of this technique for diagnostic testing.engExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license.  Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.Journal articlePIIS2666386424002352https://doi.org/10.1016/j.xcrp.2024.101975