Ohata, JunFarrukh, VohidovBall, Zachary T.2017-06-142017-06-142015Ohata, Jun, Farrukh, Vohidov and Ball, Zachary T.. "Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents." <i>Molecular BioSystems,</i> 11, (2015) Royal Society of Chemistry: 2846-2849. https://doi.org/10.1039/C5MB00510H.https://hdl.handle.net/1911/94851Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of Chemistry.Journal articlehttps://doi.org/10.1039/C5MB00510H