Royse, Madison K.Means, A. KristenCalderon, Gisele A.Kinstlinger, Ian S.He, YufangDurante, Marc R.Procopio, Adam T.Veiseh, OmidXu, Jun2022-07-262022-07-262022Royse, Madison K., Means, A. Kristen, Calderon, Gisele A., et al.. "A 3D printable perfused hydrogel vascular model to assay ultrasound-induced permeability." <i>Biomaterials Science,</i> 10, (2022) Royal Society of Chemistry: 3158-3173. https://doi.org/10.1039/D2BM00223J.https://hdl.handle.net/1911/112918The development of an in vitro model to study vascular permeability is vital for clinical applications such as the targeted delivery of therapeutics. This work demonstrates the use of a perfusion-based 3D printable hydrogel vascular model as an assessment for endothelial permeability and its barrier function. Aside from providing a platform that more closely mimics the dynamic vascular conditions in vivo, this model enables the real-time observation of changes in the endothelial monolayer during the application of ultrasound to investigate the downstream effect of ultrasound-induced permeability. We show an increase in the apparent permeability coefficient of a fluorescently labeled tracer molecule after ultrasound treatment via a custom MATLAB algorithm, which implemented advanced features such as edge detection and a dynamic region of interest, thus supporting the use of ultrasound as a non-invasive method to enhance vascular permeability for targeted drug therapies. Notably, live-cell imaging with VE-cadherin-GFP HUVECs provides some of the first real-time acquisitions of the dynamics of endothelial cell–cell junctions under the application of ultrasound in a 3D perfusable model. This model demonstrates potential as a new scalable platform to investigate ultrasound-assisted delivery of therapeutics across a cellular barrier that more accurately mimics the physiologic matrix and fluid dynamics.engJournal articlehttps://doi.org/10.1039/D2BM00223J