Narula, JatinKuchina, AnnaZhang, FangFujita, MasayaSüel, Gürol M.Igoshin, Oleg A.2016-07-062016-07-062016Narula, Jatin, Kuchina, Anna, Zhang, Fang, et al.. "Slowdown of growth controls cellular differentiation." <i>Molecular Systems Biology,</i> 12, (2016) EMBO Press: http://dx.doi.org/10.15252/msb.20156691.https://hdl.handle.net/1911/90814How can changes in growth rate affect the regulatory networks behavior and the outcomes of cellular differentiation? We address this question by focusing on starvation response in sporulating Bacillus subtilis. We show that the activity of sporulation master regulator Spo0A increases with decreasing cellular growth rate. Using a mathematical model of the phosphorelay—the network controlling Spo0A—we predict that this increase in Spo0A activity can be explained by the phosphorelay protein accumulation and lengthening of the period between chromosomal replication events caused by growth slowdown. As a result, only cells growing slower than a certain rate reach threshold Spo0A activity necessary for sporulation. This growth threshold model accurately predicts cell fates and explains the distribution of sporulation deferral times. We confirm our predictions experimentally and show that the concentration rather than activity of phosphorelay proteins is affected by the growth slowdown. We conclude that sensing the growth rates enables cells to indirectly detect starvation without the need for evaluating specific stress signals.engThis is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Journal articlehttp://dx.doi.org/10.15252/msb.20156691