Rinaldi, Mauro A.Liu, JamesEnders, Tara A.Bartel, BonnieStrader, Lucia C.2015-01-062015-01-062012Rinaldi, Mauro A., Liu, James, Enders, Tara A., et al.. "A gain-of-function mutation in IAA16 confers reduced responses to auxin and abscisic acid and impedes plant growth and fertility." <i>Plant Molecular Biology,</i> 79, no. 4-5 (2012) Springer: 359-373. http://dx.doi.org/10.1007/s11103-012-9917-y.https://hdl.handle.net/1911/78887Auxin regulates many aspects of plant development, in part, through degradation of the Aux/IAA family of transcriptional repressors. Consequently, stabilizing mutations in several Aux/IAA proteins confer reduced auxin responsiveness. However, of the 29 apparent Aux/IAA proteins in Arabidopsis thaliana, fewer than half have roles established through mutant analysis. We identified iaa16-1, a dominant gain-of-function mutation in IAA16 (At3g04730), in a novel screen for reduced root responsiveness to abscisic acid. The iaa16-1 mutation also confers dramatically reduced auxin responses in a variety of assays, markedly restricts growth of adult plants, and abolishes fertility when homozygous. We compared iaa16-1 phenotypes with those of dominant mutants defective in the closely related IAA7/AXR2, IAA14/SLR, and IAA17/AXR3, along with the more distantly related IAA28, and found overlapping but distinct patterns of developmental defects. The identification and characterization of iaa16-1 provides a fuller understanding of the IAA7/IAA14/IAA16/IAA17 clade of Aux/IAA proteins and the diverse roles of these repressors in hormone response and plant development.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.Journal articleauxin signalingabscisic acidAux/IAA gain of functionIAA16http://dx.doi.org/10.1007/s11103-012-9917-y