Cremer, MarionBrandstetter, KatharinaMaiser, AndreasRao, Suhas S.P.Schmid, Volker J.Guirao-Ortiz, MiguelMitra, NamitaMamberti, StefaniaKlein, Kyle N.Gilbert, David M.Leonhardt, HeinrichCardoso, M. CristinaAiden, Erez LiebermanHarz, HartmannCremer, Thomas2020-12-162020-12-162020Cremer, Marion, Brandstetter, Katharina, Maiser, Andreas, et al.. "Cohesin depleted cells rebuild functional nuclear compartments after endomitosis." <i>Nature Communications,</i> 11, (2020) Springer Nature: https://doi.org/10.1038/s41467-020-19876-6.https://hdl.handle.net/1911/109745Cohesin plays an essential role in chromatin loop extrusion, but its impact on a compartmentalized nuclear architecture, linked to nuclear functions, is less well understood. Using live-cell and super-resolved 3D microscopy, here we find that cohesin depletion in a human colon cancer derived cell line results in endomitosis and a single multilobulated nucleus with chromosome territories pervaded by interchromatin channels. Chromosome territories contain chromatin domain clusters with a zonal organization of repressed chromatin domains in the interior and transcriptionally competent domains located at the periphery. These clusters form microscopically defined, active and inactive compartments, which likely correspond to A/B compartments, which are detected with ensemble Hi-C. Splicing speckles are observed nearby within the lining channel system. We further observe that the multilobulated nuclei, despite continuous absence of cohesin, pass through S-phase with typical spatio-temporal patterns of replication domains. Evidence for structural changes of these domains compared to controls suggests that cohesin is required for their full integrity.engThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.Journal articles41467-020-19876-6https://doi.org/10.1038/s41467-020-19876-6