Kyrish, MatthewTkaczyk, Tomasz S.2013-04-292013-04-292013Kyrish, Matthew and Tkaczyk, Tomasz S.. "Achromatized endomicroscope objective for optical biopsy." <i>Journal of Biomedical Optics Express,</i> 4, no. 2 (2013) Optical Society of America: http://dx.doi.org/10.1364/BOE.4.000287.https://hdl.handle.net/1911/71012Currently, researchers and clinicians lack achromatized endomicroscope objectives that are as narrow as biopsy needles. We present a proof-of-concept prototype that validates the optical design of an NA0.4 objective. The objective, built with plastic lenses, has a 0.9 mm clear aperture and is achromatized from 452 nm to 623 nm. The objective’s measured Strehl ratio is 0.74 ± 0.05 across a 250 μm FOV. We perform optical sectioning via structured illumination through the objective while capturing fluorescence images of breast carcinoma cells stained with proflavine and cresyl violet. This technology has the potential to improve optical biopsies and provide the next step forward in cancer diagnostics.engArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.Journal articlehttp://dx.doi.org/10.1364/BOE.4.000287