Castillo-Hair, Sebastian M.Fujita, MasayaIgoshin, Oleg A.Tabor, Jeffrey J.2019-08-142019-08-142019Castillo-Hair, Sebastian M., Fujita, Masaya, Igoshin, Oleg A., et al.. "An Engineered B. subtilis Inducible Promoter System with over 10 000-Fold Dynamic Range." <i>ACS Synthetic Biology,</i> 8, no. 7 (2019) American Chemical Society: 1673-1678. https://doi.org/10.1021/acssynbio.8b00469.https://hdl.handle.net/1911/106241Bacillus subtilis is the leading model Gram-positive bacterium, and a widely used chassis for industrial protein production. However, B. subtilis research is limited by a lack of inducible promoter systems with low leakiness and high dynamic range. Here, we engineer an inducible promoter system based on the T7 RNA Polymerase (T7 RNAP), the lactose repressor LacI, and the chimeric promoter PT7lac, integrated as a single copy in the B. subtilis genome. In the absence of IPTG, LacI strongly represses T7 RNAP and PT7lac and minimizes leakiness. Addition of IPTG derepresses PT7lac and simultaneously induces expression of T7RNAP, which results in very high output expression. Using green fluorescent and β-galactosidase reporter proteins, we estimate that this LacI-T7 system can regulate expression with a dynamic range of over 10 000, by far the largest reported for an inducible B. subtilis promoter system. Furthermore, LacI-T7 responds to similar IPTG concentrations and with similar kinetics as the widely used Phy-spank IPTG-inducible system, which we show has a dynamic range of at most 300 in a similar genetic context. Due to its superior performance, our LacI-T7 system should have broad applications in fundamental B. subtilis biology studies and biotechnology.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.Journal articleIPTGT7 RNA polymeraseinducible promoter systemprotein expressionhttps://doi.org/10.1021/acssynbio.8b00469