Hilton, Isaac BBennett, Matthew2023-08-092023-052023-04-20May 2023Escobar Galdamez, Mario D. "Design and Benchmarking of CRISPR/Cas Tools for Transcriptional Regulation in Human Cells." (2023) Diss., Rice University. <a href="https://hdl.handle.net/1911/115139">https://hdl.handle.net/1911/115139</a>.https://hdl.handle.net/1911/115139EMBARGO NOTE: This item is embargoed until 2024-05-01CRISPR/Cas systems have revolutionized biomedical research by providing easily programmable nucleic acid-targeting proteins. However, the discovery of different CRISPR systems in various organisms and their rapid proliferation in research settings have crowded the field and ultimately complicated the use of CRISPR tools. During my research, I investigated how different CRISPR/Cas9 design strategies impact the efficiency of these tools. Additionally, I quantified and compared the efficacy of CRISPR/Cas systems from a variety of different species for genome editing and transcriptional control. Finally, I leveraged these optimized design parameters to create a new synthetic system for controlling the generation of new mitochondria within living human cells.application/pdfengCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.CRISPRCRISPRaEpigeneticsThesis2023-08-09