Ware, Kathryn E.Gilja, ShiveeXu, ShenghanShetler, SamanthaJolly, Mohit K.Wang, XueyangDewitt, Suzanne BartholfHish, Alexander J.Jordan, SarahEward, WilliamLevine, HerbertArmstrong, Andrew J.Somarelli, Jason A.2017-08-112017-08-112017Ware, Kathryn E., Gilja, Shivee, Xu, Shenghan, et al.. "Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells." <i>Journal of Visualized Experiments,</i> 122, (2017) JoVE: https://doi.org/10.3791/55520.https://hdl.handle.net/1911/96649We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.engArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.Journal articlehttps://doi.org/10.3791/55520