Cooper, DavidUhm, HeuiTauzin, Lawrence J.Poddar, NiteshLandes, Christy F.2014-10-062014-10-062013Cooper, David, Uhm, Heui, Tauzin, Lawrence J., et al.. "Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems." <i>ChemBioChem,</i> 14, no. 9 (2013) John Wiley & Sons, Inc.: 1075-1080. http://dx.doi.org/10.1002/cbic.201300030.https://hdl.handle.net/1911/77399We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimesengThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrightedby John Wiley & Sons Ltd.Journal articleDNAfluorophoreFRETphotobleachsingle-molecule studieshttp://dx.doi.org/10.1002/cbic.201300030