Wang, LushunWang, ShichaoTang, JuanEspinoza, Vanessa B.Loredo, AxelTian, ZeruWeisman, R. BruceXiao, Han2022-01-072022-01-072021Wang, Lushun, Wang, Shichao, Tang, Juan, et al.. "Oxime as a general photocage for the design of visible light photo-activatable fluorophores." <i>Chemical Science,</i> 12, (2021) Royal Society of Chemistry: 15572-15580. https://doi.org/10.1039/D1SC05351E.https://hdl.handle.net/1911/111914Photoactivatable fluorophores have been widely used for tracking molecular and cellular dynamics with subdiffraction resolution. In this work, we have prepared a series of photoactivatable probes using the oxime moiety as a new class of photolabile caging group in which the photoactivation process is mediated by a highly efficient photodeoximation reaction. Incorporation of the oxime caging group into fluorophores results in loss of fluorescence. Upon light irradiation in the presence of air, the oxime-caged fluorophores are oxidized to their carbonyl derivatives, restoring strong fluorophore fluorescence. To demonstrate the utility of these oxime-caged fluorophores, we have created probes that target different organelles for live-cell confocal imaging. We also carried out photoactivated localization microscopy (PALM) imaging under physiological conditions using low-power light activation in the absence of cytotoxic additives. Our studies show that oximes represent a new class of visible-light photocages that can be widely used for cellular imaging, sensing, and photo-controlled molecular release.engThis Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported LicenceJournal articled1sc05351ehttps://doi.org/10.1039/D1SC05351E