2015-05-042015-05-042013-07-16Segueilha, Laurent, San, Ka-Yiu, Bennett, George N. and Martinez, Irene, "Large scale microbial culture method." Patent US8486686B2. issued 2013-07-16. Retrieved from https://hdl.handle.net/1911/80129.https://hdl.handle.net/1911/80129A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having13 ppengUtility patentUS8486686B2