Kinard, Lucas A.Kasper, F. KurtisMikos, Antonios G.2013-08-202013-08-202012Kinard, Lucas A., Kasper, F. Kurtis and Mikos, Antonios G.. "Synthesis of oligo(poly(ethylene glycol) fumarate)." <i>Nature Protocols,</i> 7, no. 6 (2012) Nature Publishing Group: 1219-1227. http://dx.doi.org/10.1038/nprot.2012.055.https://hdl.handle.net/1911/71749This protocol describes the synthesis of oligo(poly(ethylene glycol) fumarate) (OPF) (1-35 kDa)(a polymer useful for tissue engineering applications) by a one-pot reaction of poly(ethylene glycol) (PEG) and fumaryl chloride. The procedure involves three parts: dichloromethane and PEG are first dried; the reaction step follows in which fumaryl chloride and triethylamine are added dropwise to a solution of PEG in dichloromethane; and finally the product solution is filtered to remove byproduct salt, and the OPF product is twice crystallized, washed, and dried under vacuum. The reaction is affected by PEG molecular weight and reactant molar ratio. The OPF product is cross-linked by radical polymerization by either a thermally induced or UV-induced radical initiator, and the physical properties of the OPF oligomer and resulting cross-linked hydrogel are easily tailored by varying PEG molecular weight. OPF hydrogels are injectable, polymerize in situ, and undergo biodegradation by hydrolysis of ester bonds. The expected time required to complete this protocol is 6 d.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Nature Publishing Group.Journal articleoligo(poly(ethylene glycol) fumarate)OPFpolymertissue engineeringpolymer synthesisradical polymerizationhydrogelPEGhttp://dx.doi.org/10.1038/nprot.2012.055