Lusk, Savannah J.McKinney, AndrewHunt, Patrick J.Fahey, Paul G.Patel, JayChang, AndersenSun, Jenny J.Martinez, Vena K.Zhu, Ping JunEgbert, Jeremy R.Allen, GeneveraJiang, XiaolongArenkiel, Benjamin R.Tolias, Andreas S.Costa-Mattioli, MauroRay, Russell S.2022-03-242022-03-242022Lusk, Savannah J., McKinney, Andrew, Hunt, Patrick J., et al.. "A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping." <i>BMC Biology,</i> 20, (2022) Springer Nature: https://doi.org/10.1186/s12915-022-01227-0.https://hdl.handle.net/1911/112058The functional understanding of genetic interaction networks and cellular mechanisms governing health and disease requires the dissection, and multifaceted study, of discrete cell subtypes in developing and adult animal models. Recombinase-driven expression of transgenic effector alleles represents a significant and powerful approach to delineate cell populations for functional, molecular, and anatomical studies. In addition to single recombinase systems, the expression of two recombinases in distinct, but partially overlapping, populations allows for more defined target expression. Although the application of this method is becoming increasingly popular, its experimental implementation has been broadly restricted to manipulations of a limited set of common alleles that are often commercially produced at great expense, with costs and technical challenges associated with production of intersectional mouse lines hindering customized approaches to many researchers. Here, we present a simplified CRISPR toolkit for rapid, inexpensive, and facile intersectional allele production.engThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Journal articles12915-022-01227-0https://doi.org/10.1186/s12915-022-01227-0