2019-11-062019-11-062019-10-22Gonzalez, Ramon, Clomburg, James M. and Vick, Jacob E., "Type II fatty acid synthesis enzymes in reverse β-oxidation." Patent US10450593B2. issued 2019-10-22. Retrieved from <a href="https://hdl.handle.net/1911/107616">https://hdl.handle.net/1911/107616</a>.https://hdl.handle.net/1911/107616This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis (“FAS”) pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.43engUtility patentUS10450593B2