2023-02-082023-02-082023-01-31Tour, James M., Pal, Robert, García, López Victor and Nilewski, Lizanne, "Mechanical opening of lipid bilayers by molecular nanomachines." Patent US11565003B2. issued 2023-01-31. Retrieved from <a href="https://hdl.handle.net/1911/114453">https://hdl.handle.net/1911/114453</a>.https://hdl.handle.net/1911/114453Embodiments of the present disclosure pertain to methods of opening a lipid bilayer by associating the lipid bilayer with a molecule that includes a moving component capable of moving (e.g., rotating) in response to an external stimulus; and exposing the molecule to an external stimulus before, during or after associating the molecule with the lipid bilayer. The exposing causes the moving component of the molecule to move and thereby open the lipid bilayer (e.g., by pore formation). The external stimuli may include an energy source, such as ultraviolet light. The opened lipid bilayer may be a component of cell membranes in vitro or in vivo. The opening of the lipid bilayer may allow for the passage of various materials (e.g., active agents, such as peptide-based drugs) through the lipid bilayer and into cells. Additional embodiments of the present disclosure pertain to the aforementioned molecules for opening lipid bilayers.70engUtility patentUS11565003B2