Singh, ShanteriKim, YoungchangWang, FengbinBigelow, LanceEndres, MichaelKharel, Madan K.Babnigg, GyorgyBingman, Craig A.Joachimiak, AndrzejThorson, Jon S.Phillips, George N.Jr.2016-10-212016-10-212015Singh, Shanteri, Kim, Youngchang, Wang, Fengbin, et al.. "Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis." <i>Proteins: Structure, Function, and Bioinformatics,</i> 83, no. 8 (2015) Wiley: 1547-1554. http://dx.doi.org/10.1002/prot.24844.https://hdl.handle.net/1911/91979AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.Journal articlex-ray crystallographycarbohydrateindolocarbazolenatural productsugar nucleotidetransaminationhttp://dx.doi.org/10.1002/prot.24844