Lukianova-Hleb, Ekaterina Y.Wagner, Daniel S.Brenner, Malcolm K.Lapotko, Dmitri O.2015-01-062015-01-062012Lukianova-Hleb, Ekaterina Y., Wagner, Daniel S., Brenner, Malcolm K., et al.. "Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles." <i>Biomaterials,</i> 33, no. 21 (2012) Elsevier: 5441-5450. http://dx.doi.org/10.1016/j.biomaterials.2012.03.077.https://hdl.handle.net/1911/78889Optimal cell therapies require efficient, selective and rapid delivery of molecular cargo into target cells without compromising their viability. Achieving these goals exﾠvivo in bulk heterogeneous multi-cell systems such as human grafts is impeded by low selectivity and speed of cargo delivery and by significant damage to target and non-target cells. We have developed a cell level approach for selective and guided transmembrane injection of extracellular cargo into specific target cells using transient plasmonic nanobubbles (PNB) as cell-specific nano-injectors. As a technical platform for this method we developed a laser flow cell processing system. The PNB injection method and flow system were tested in heterogeneous cell suspensions of target and non-target cells for delivery of Dextran-FITC dye into squamous cell carcinoma HN31 cells and transfection of human T-cells with a green fluorescent protein-encoding plasmid. In both models the method demonstrated single cell type selectivity, high efficacy of delivery (96% both for HN31 cells T-cells), speed of delivery (nanoseconds) and viability of treated target cells (96% for HN31 cells and 75% for T-cells). The PNB injection method may therefore be beneficial for real time processing of human grafts without removal of physiologically important cells.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.Journal articleDNAgene transferlasergoldnanoparticlehttp://dx.doi.org/10.1016/j.biomaterials.2012.03.077