Shuang, BoByers, Chad P.Kisley, LydiaWang, Lin-YungZhao, JuliaMorimura, HiroyukiLink, StephanLandes, Christy F.2014-12-152014-12-152013Shuang, Bo, Byers, Chad P., Kisley, Lydia, et al.. "Improved Analysis for Determining Diffusion Coefficients from Short, Single-Molecule Trajectories with Photoblinking." <i>Langmuir,</i> 29, no. 1 (2013) American Chemical Society: 228-234. http://dx.doi.org/10.1021/la304063j.https://hdl.handle.net/1911/78758Two Maximum Likelihood Estimation (MLE) methods were developed for optimizing the analysis of single-molecule trajectories that include phenomena such as experimental noise, photoblinking, photobleaching, and translation or rotation out of the collection plane. In particular,short, single-molecule trajectories with photoblinking were studied, and our method was compared with existing analytical techniques applied to simulated data. The optimal method for various experimental cases was established, and the optimized MLE method was applied to a real experimental system: single-molecule diffusion of fluorescent molecular machines known as nanocars.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.Journal articlehttp://dx.doi.org/10.1021/la304063j