Veiseh, Omid2022-09-262022-09-262022-052022-04-22May 2022Lu, Alexander. "mplantable Cell Based Therapy for Treatment of Inflammatory Heart and Lung Conditions." (2022) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/113352">https://hdl.handle.net/1911/113352</a>.https://hdl.handle.net/1911/113352In this work, we explore utilizing encapsulated engineered cells for targeted release of anti-inflammatory cytokines. A plasmid encoding the cytokine hIL-10 and suicide system iCasp9 was created and inserted into ARPE-19 cells. After integration, cells were assayed for hIL-10 production and response to an iCasp9 activating molecule. To better understand clinical translatability of a capsule based delivery system, two surgical approaches to delivery of capsules were tested on cadaveric porcine hearts and cadaveric mice. hIL-10 cells showed variable hIL-10 production over 24 hours, ranging from 0.3 - 5.2 pg/cell/24hr. Cells exposed to 1nM of AP1903, 10% of previously reported doses, demonstrated significant cell death over 24 hours. Existing surgical methods involving placement of capsules ultimately proved unsuitable for clinical use. This work serves as an initial proof of concept for treating inflammatory conditions using biologically and physically targeted therapies.application/pdfengCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.cell engineeringanti-inflammatoryencapsulationcytokinesThesis2022-09-26