Wang, ChunyanBae, Jin H.Zhang, David Yu2016-06-062016-06-062016Wang, Chunyan, Bae, Jin H. and Zhang, David Yu. "Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis." <i>Nature Communications,</i> 7, (2016) Nature Publishing Group: http://dx.doi.org/10.1038/ncomms10319.https://hdl.handle.net/1911/90448DNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG° of DNA motifs at temperatures and buffer conditions of interest, with significantly better accuracy (6- to 14-fold lower s.e.) than prior methods. The equilibrium constant of a reaction with thermodynamics closely approximating that of a desired motif is numerically calculated from directly observed reactant and product equilibrium concentrations; a DNA catalyst is designed to accelerate equilibration. We measured the ΔG° of terminal fluorophores, single-nucleotide dangles and multinucleotide dangles, in temperatures ranging from 10 to 45 °C.engThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the articleﾒs Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.Journal articlehttp://dx.doi.org/10.1038/ncomms10319