2015-05-042015-05-042010-09-28Olson, John S. and Phillips, George N., Jr., "Increasing the stability of recombinant adult human apohemoglobin." Patent US7803912B2. issued 2010-09-28. Retrieved from https://hdl.handle.net/1911/80034.https://hdl.handle.net/1911/80034The disclosure relates to recombinant adult human apohemoglobin (apo-rHb) in which the stability has been increased by replacement of an amino acid with a counterpart from another organism, such as a deep sea diving mammal. This mutated apo-rHb may be more stable and/or give higher production yields than unmutated adult human apo-rHb. The mutated apo-rHb may be produced in microorganisms, such as E. coli or yeast cells, or animal erythroid cells. Some apo-rHb of the present disclosure may be used as part of a blood substitute.20 ppengUtility patentUS7803912B2