Shuang, BoChen, JixinKisley, LydiaLandes, Christy F.2015-03-162015-03-162014Shuang, Bo, Chen, Jixin, Kisley, Lydia, et al.. "Troika of single particle tracking programing: SNR enhancement, particle identification, and mapping." <i>Physical Chemistry Chemical Physics,</i> 16, no. 2 (2014) Royal Society of Chemistry: 624-634. http://dx.doi.org/10.1039/C3CP53968G.https://hdl.handle.net/1911/79344Single particle tracking (SPT) techniques provide a microscopic approach to probe in vivo and in vitro structure and reactions. Automatic analysis of SPT data with high efficiency and accuracy spurs the development of SPT algorithms. In this perspective, we review a range of available techniques used in SPT analysis programs. In addition, we present an example SPT program step-by-step to provide a guide so that researchers can use, modify, and/or write a SPT program for their own purposes.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of ChemistryJournal articlehttp://dx.doi.org/10.1039/C3CP53968G